Vol. 5 No. 2 (2021)
Articles

A Dynamic Approach to Detect Anticancer Activity of Chitiniphilus shinanonensis

Published 2021-08-07

Abstract

Marine organisms are of primary interest owing to their complex cell life and their bioprocess potential. Chitin is one of the most abundant polysaccharides found in nature after cellulose. Chitinases can be used in the production of functional chitin-oligosaccharides; in making of single cell protein and in the preparation of fungal protoplasts, culture medium of cultured cancer cell. Keeping in view of this, chitin degrading ability of five marine organisms were evaluated in the present study. The most potent organism (M5) was further subjected to media optimisation to enhance the secondary metabolite production. The effective bioactive extract was also determined by trying different solvent extractions. The anti-bacterial and anti-cancer effects of the secondary metabolites present in the pure extract also determined and showed potent responses. The structure of active compounds was determined as Asiatic acid and triterpene which are known to exhibit potential anti-cancer properties. The synergistic effect of Asiatic acid and triterpene bioactive compounds isolated from marine organism (M2) on anti-bacterial and anti-cancer effects are of potential significance in both future bioprocess and therapeutic approaches.